利用毛细作用直接装载细胞
无需人工控制冷冻液体积
将复温和稀释简化为一个步骤,
利用空气热膨胀原理自动排出细胞
*玻化冻存管现已取得中国NMPA注册证、欧盟CE认证及美国上市许可,可在符合条件的国家及地区出售。
请联系我们以获得更多的相关信息
分步操作:准确指导整个玻璃化冷冻-复苏过程,内置秒表功能;
交互式、个性化的信息:可自由选择每个步骤;
直接与首席科学家Gábor Vajta教授沟通获取技术支持
内毒素 < 0.5EU/套
鼠胚囊胚扩张率 ≥ 80%
无菌测试:通过
pH值 7.2-7.6
内毒素 < 0.25EU/mL
鼠胚囊胚扩张率 ≥ 80%
无菌测试:通过
产品 | 货号 | 规格 | 有效期 |
---|---|---|---|
玻化冻存管 | V001001 | 5支/盒 | 24个月 |
玻璃化冷冻液 | V002001 | 基础液 1.0mL x1 冷冻液1 1.0mL x1 冷冻液2 1.0mL x1 | 12个月 |
V002002 | 基础液 4.5mL x1 冷冻液1 4.5mL x1 冷冻液2 4.5mL x2 | ||
V002004 | 基础液 2.0mL x1 冷冻液1 2.0mL x2 冷冻液2 2.0mL x2 | ||
V002005 | 基础液 4.8mL x2 冷冻液1 4.8mL x2 冷冻液2 4.8mL x2 | ||
玻璃化解冻液 | V003001 | 基础液 1.8mL x1 解冻液1 1.5mL x2 解冻液2 1.0mL x1 | 12个月 |
V003002 | 基础液 4.5mL x2 解冻液1 4.5mL x1 解冻液2 4.5mL x1 | ||
V003004 | 基础液 2.0mL x3 解冻液1 2.0mL x5 解冻液2 2.0mL x2 | ||
V003005 | 基础液 4.8mL x2 解冻液1 4.8mL x2 解冻液2 4.8mL x1 | ||
牙钳 | VOPS01 | ─ | ─ |
热封钳 | VOPS02 | ─ | ─ |
封口剪 | VOPS03 | ─ | ─ |
液氮盒 | VOPS04 | 一个/箱 | ─ |
1. Vajta G., Holm P., Kumayama M., Booth P.J., Jacobsen H., Greve T., Callesen H. Open Pulled Straw (OPS) Vitrification: A New Way to Reduce Cryoinjuries of Bovine Ova and Embryos. Molecular Reproduction and Development. 1999; 51(1): 53-58. DOI: 10.1002/(sici)1098-2795(199809)51:1<53::aid-mrd6>3.0.co;2-v.
2. Kuleshova, L., Gianaroli, L., Magli, C., Ferraretti, A., Trounson, A. Birth following vitrification of a small number of human oocytes: Case Report. Human Reproduction. 1999; 14(12): 3077–3079. DOI:10.1093/humrep/14.12.3077.
3. Vieira, A., Mezzalira, A., Barbieri, D., Lehmkuhl, R., Rubin, M. I., Vajta, G. Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology. 2002; 45(1): 91–94. DOI: 10.1016/s0011-2240(02)00109-8.
4. Reubinoff, B. E., Pera, M. F., Vajta, G., Trounson, A. O. Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Human Reproduction. 2001; 16(10): 2187–2194. DOI:10.1093/humrep/16.10.2187.
5. Vajta G. Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reproductive Bio-Medicine Online. 2006; 12: 779-796. DOI: 10.1016/s1472-6483(10)61091-7.
6. Vajta G. Vitrification in human and domestic animal embryology: work in progress. Reproduction, Fertility and Development. 2012; 25(5): 719-727. DOI: 10.1071/RD12118.
7. Vajta G., Reichart A., Baldi F. U., Rienzi L. From a back up technology to a strategy-outlining approach: the success story of cryopreservation. Expert Rev. Obstet. Gynecol. 2013; 8(2): 181-190. DOI: 10.1586/eog.12.80.
8. Munck N. D, Vajta G. Safety and efficiency of oocyte vitrification, Cryobiology. 2017; 78: 119-127. DOI: 10.1016/j.cryobi-ol.2017.07.009.
9. Vajta G. Vitrification in ART: past, present and future. Theriogenology. 2020; 150: 276-279. DOI:1016/j.theriogenolo-gy.2020.01.057.
VitaVitro 玻璃化冷冻、解冻操作说明